| 质粒名称: | pLenti PGK V5-LUC Neo (w623-2) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 21471 |
| 存储实验室: | Eric Campeau |
|
载体骨架基本信息 |
|
| 载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
| 载体出品公司: | -- |
| 空载体大小: | 7334 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒，荧光素酶 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | V5-LUC,Firefly Luciferase |
| 物种来源: | Firefly |
| 插入大小: | 1776 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | V5（插入件上的N端子） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Bam HI(未破坏) |
| 3’克隆位点: | Xba I (未破坏) |
| 5’测序引物 | PGKforw |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti PGK V5-LUC Neo (w623-2) was a gift from Eric Campeau (Addgene plasmid # 21471 ; http://n2t.net/addgene:21471 ; RRID:Addgene_21471) |
| 参考文献部分: | A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pLenti PGK V5-LUC Neo (w623-2) 9916 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION 3rd gen lentiviral V5-Luciferase expression vector, PGK promoter,
Neo.
ACCESSION .
VERSION .
KEYWORDS pLenti PGK V5-LUC Neo (w623-2)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9916)
AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO,
Campisi J, Yaswen P, Cooper PK, Kaufman PD.
TITLE A Versatile Viral System for Expression and Depletion of Proteins in
Mammalian Cells
JOURNAL PLoS One. 2009 Aug 6;4(8):e6529.
PUBMED 19657394
REFERENCE 2 (bases 1 to 9916)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9916
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 98..115
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 232..253
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 268..298
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 306..322
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 311..333
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 330..346
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 330..346
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 365..385
/label=T3
/note="T3 promoter, forward primer"
promoter 367..385
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 413..639
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 640..820
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 867..992
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1485..1718
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1903..1947
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2214..2331
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2377..2887
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 2806..2824
/label=hPGK-F
/note="Human PGK promoter, forward primer"
protein_bind 2942..2966
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 2978..3019
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
regulatory 3089..3098
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3095..4747
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(3161..3179)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
protein_bind complement(4754..4778)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 4809..5397
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4862..4882)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5280..5291)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 5440..5939
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(5475..5498)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 5872..5891
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 5953..6756
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6016..6035)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 6626..6645
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
LTR 6847..7080
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 7152..7273
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7189..7208)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7243..7262
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 7313..7448
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7375..7394
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(7468..7487)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(7469..7487)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(7497..7514)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(7497..7513)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7506..7528)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 7655..8110
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7742..7761)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7952..7973
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 8136..8240
/gene="bla"
/label=AmpR promoter
CDS 8241..9101
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8459..8478)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9272..9860
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9761..9780
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
361 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
421 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
481 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
541 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
601 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
661 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
721 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
781 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
841 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
901 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
961 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
1021 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
1081 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
1141 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
1201 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
1261 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
1321 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
1381 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
1441 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
1501 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
1561 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
1621 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
1681 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
1741 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
1801 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
1861 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
1921 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
1981 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
2041 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
2101 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
2161 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa
2221 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
2281 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac
2341 gagactagcc tcgagaagct tgatatcgaa ttccacgggg ttggggttgc gccttttcca
2401 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag
2461 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt
2521 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg
2581 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac
2641 cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc
2701 caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga
2761 ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg
2821 caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc
2881 tccccagggg gatccaccgg ttcgtcgact agtccagtgt ggtggaattc tgcagatatc
2941 aacaagtttg tacaaaaaag caggctccac catggaaggt aagcctatcc ctaaccctct
3001 cctcggtctc gattctacgg gatccatggg aaccaattca gtccctaggc ttttgcaaaa
3061 agcttggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa acataaagaa
3121 aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc
3181 tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt
3241 ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg
3301 atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt
3361 tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta
3421 taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc
3481 caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat
3541 tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac
3601 atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct tcgataggga
3661 caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta aaggtgtcgc
3721 tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta tttttggcaa
3781 tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat
3841 gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga
3901 agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc tgctggtgcc
3961 aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt
4021 acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag cggttgccaa
4081 gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta catcagctat
4141 tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt
4201 tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc aaagaggcga
4261 actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa
4321 cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga
4381 cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag gctatcaggt
4441 ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg acgcaggtgt
4501 cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca
4561 cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc
4621 gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa
4681 actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagatcgc
4741 cgtgtaattc tagacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc
4801 cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
4861 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
4921 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
4981 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
5041 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
5101 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
5161 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
5221 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
5281 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
5341 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
5401 gaattctgca gatatccgga ctagtgatct aattctaccg ggtaggggag gcgcttttcc
5461 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
5521 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
5581 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
5641 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
5701 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
5761 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
5821 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
5881 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
5941 cctgcagcca atatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc
6001 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
6061 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
6121 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
6181 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
6241 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
6301 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
6361 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
6421 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
6481 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
6541 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
6601 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
6661 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
6721 atcgccttct atcgccttct tgacgagttc ttctgagggg atccgtcgag ggcccggtac
6781 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
6841 ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg
6901 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
6961 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
7021 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
7081 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
7141 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
7201 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
7261 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
7321 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
7381 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
7441 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
7501 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
7561 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
7621 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
7681 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
7741 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
7801 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
7861 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
7921 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
7981 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
8041 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
8101 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
8161 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
8221 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
8281 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
8341 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
8401 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
8461 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
8521 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
8581 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
8641 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
8701 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
8761 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
8821 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
8881 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
8941 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
9001 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
9061 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
9121 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
9181 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
9241 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
9301 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
9361 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
9421 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
9481 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
9541 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
9601 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
9661 agctatgaga aagcgccacg cttcccgaag ggagaaaggt ggacaggtat ccggtaagcg
9721 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
9781 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
9841 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
9901 gctggccttt tgctca
//
